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anti-o4 microbeads, human, mouse  (Miltenyi Biotec)


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    Miltenyi Biotec anti-o4 microbeads, human, mouse
    Anti O4 Microbeads, Human, Mouse, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 87 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 87 article reviews
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    95/100 stars

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    anti-o4 microbeads, human, mouse  (Miltenyi Biotec)
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    Anti O4 Microbeads, Human, Mouse, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti-o4 microbeads, human, mouse/product/Miltenyi Biotec
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    anti o4 microbeads  (Miltenyi Biotec)
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    A , experimental design. Inflammatory demyelination of experimental autoimmune encephalomyelitis (EAE) was induced in adult mice via MOG immunization (CFA as control) at day 0 (D0), with pertussis administered i.p. on D0 and D2. RNA-seq was performed on whole spinal cords ( B ) and MACS-purified glial populations ( C-F ) at D30-D35. OLs, oligodendrocytes; Astro, astrocytes; MG, microglia. B , volcano plot of differentially expressed genes (DEGs) in spinal cord of MOG/EAE (n=4) vs. CFA mice (n=3) at D35. Top10 DEGs are labeled with gene symbols and FPKM values. C , schematic of magnetic beads-assisted cell sorting (MACS) of spinal cord glia at D30. OL, Astro, and myeloid cells (including MG) were isolated using <t>anti-O4,</t> anti-ACSA2, and anti-CD11b magnetic beads, respectively. D , purity validation of sorted population using representative markers Plp1 (OL), Tmem119 (MG), and Aldh1l1 (Astro). Expression is shown as TPM (transcripts per million). E , volcano plot of DEGs in MOG_OL versus CFA_OL. Serpina3n is highlighted in red. F , TPM expression of Serpina3n , Ctsd , Lgals3 , and Gpnmb in OLs, Astro, and MG from MOG/EAE and CFA mice. Serpina3n is specifically upregulated in OL, while Ctsd , Lgals3 , and Gpnmb are enriched and upregulated in MG.
    Anti O4 Microbeads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti o4 microbeads/product/Miltenyi Biotec
    Average 95 stars, based on 1 article reviews
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    o4 microbeads  (Miltenyi Biotec)
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    A , experimental design. Inflammatory demyelination of experimental autoimmune encephalomyelitis (EAE) was induced in adult mice via MOG immunization (CFA as control) at day 0 (D0), with pertussis administered i.p. on D0 and D2. RNA-seq was performed on whole spinal cords ( B ) and MACS-purified glial populations ( C-F ) at D30-D35. OLs, oligodendrocytes; Astro, astrocytes; MG, microglia. B , volcano plot of differentially expressed genes (DEGs) in spinal cord of MOG/EAE (n=4) vs. CFA mice (n=3) at D35. Top10 DEGs are labeled with gene symbols and FPKM values. C , schematic of magnetic beads-assisted cell sorting (MACS) of spinal cord glia at D30. OL, Astro, and myeloid cells (including MG) were isolated using <t>anti-O4,</t> anti-ACSA2, and anti-CD11b magnetic beads, respectively. D , purity validation of sorted population using representative markers Plp1 (OL), Tmem119 (MG), and Aldh1l1 (Astro). Expression is shown as TPM (transcripts per million). E , volcano plot of DEGs in MOG_OL versus CFA_OL. Serpina3n is highlighted in red. F , TPM expression of Serpina3n , Ctsd , Lgals3 , and Gpnmb in OLs, Astro, and MG from MOG/EAE and CFA mice. Serpina3n is specifically upregulated in OL, while Ctsd , Lgals3 , and Gpnmb are enriched and upregulated in MG.
    O4 Microbeads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti o4 apc antibody  (Miltenyi Biotec)
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    A , experimental design. Inflammatory demyelination of experimental autoimmune encephalomyelitis (EAE) was induced in adult mice via MOG immunization (CFA as control) at day 0 (D0), with pertussis administered i.p. on D0 and D2. RNA-seq was performed on whole spinal cords ( B ) and MACS-purified glial populations ( C-F ) at D30-D35. OLs, oligodendrocytes; Astro, astrocytes; MG, microglia. B , volcano plot of differentially expressed genes (DEGs) in spinal cord of MOG/EAE (n=4) vs. CFA mice (n=3) at D35. Top10 DEGs are labeled with gene symbols and FPKM values. C , schematic of magnetic beads-assisted cell sorting (MACS) of spinal cord glia at D30. OL, Astro, and myeloid cells (including MG) were isolated using <t>anti-O4,</t> anti-ACSA2, and anti-CD11b magnetic beads, respectively. D , purity validation of sorted population using representative markers Plp1 (OL), Tmem119 (MG), and Aldh1l1 (Astro). Expression is shown as TPM (transcripts per million). E , volcano plot of DEGs in MOG_OL versus CFA_OL. Serpina3n is highlighted in red. F , TPM expression of Serpina3n , Ctsd , Lgals3 , and Gpnmb in OLs, Astro, and MG from MOG/EAE and CFA mice. Serpina3n is specifically upregulated in OL, while Ctsd , Lgals3 , and Gpnmb are enriched and upregulated in MG.
    Anti O4 Apc Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti o4 microbeads miltenyi biotec  (Miltenyi Biotec)
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    A , experimental design. Inflammatory demyelination of experimental autoimmune encephalomyelitis (EAE) was induced in adult mice via MOG immunization (CFA as control) at day 0 (D0), with pertussis administered i.p. on D0 and D2. RNA-seq was performed on whole spinal cords ( B ) and MACS-purified glial populations ( C-F ) at D30-D35. OLs, oligodendrocytes; Astro, astrocytes; MG, microglia. B , volcano plot of differentially expressed genes (DEGs) in spinal cord of MOG/EAE (n=4) vs. CFA mice (n=3) at D35. Top10 DEGs are labeled with gene symbols and FPKM values. C , schematic of magnetic beads-assisted cell sorting (MACS) of spinal cord glia at D30. OL, Astro, and myeloid cells (including MG) were isolated using <t>anti-O4,</t> anti-ACSA2, and anti-CD11b magnetic beads, respectively. D , purity validation of sorted population using representative markers Plp1 (OL), Tmem119 (MG), and Aldh1l1 (Astro). Expression is shown as TPM (transcripts per million). E , volcano plot of DEGs in MOG_OL versus CFA_OL. Serpina3n is highlighted in red. F , TPM expression of Serpina3n , Ctsd , Lgals3 , and Gpnmb in OLs, Astro, and MG from MOG/EAE and CFA mice. Serpina3n is specifically upregulated in OL, while Ctsd , Lgals3 , and Gpnmb are enriched and upregulated in MG.
    Anti O4 Microbeads Miltenyi Biotec, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    o4 apc  (Miltenyi Biotec)
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    A , experimental design. Inflammatory demyelination of experimental autoimmune encephalomyelitis (EAE) was induced in adult mice via MOG immunization (CFA as control) at day 0 (D0), with pertussis administered i.p. on D0 and D2. RNA-seq was performed on whole spinal cords ( B ) and MACS-purified glial populations ( C-F ) at D30-D35. OLs, oligodendrocytes; Astro, astrocytes; MG, microglia. B , volcano plot of differentially expressed genes (DEGs) in spinal cord of MOG/EAE (n=4) vs. CFA mice (n=3) at D35. Top10 DEGs are labeled with gene symbols and FPKM values. C , schematic of magnetic beads-assisted cell sorting (MACS) of spinal cord glia at D30. OL, Astro, and myeloid cells (including MG) were isolated using <t>anti-O4,</t> anti-ACSA2, and anti-CD11b magnetic beads, respectively. D , purity validation of sorted population using representative markers Plp1 (OL), Tmem119 (MG), and Aldh1l1 (Astro). Expression is shown as TPM (transcripts per million). E , volcano plot of DEGs in MOG_OL versus CFA_OL. Serpina3n is highlighted in red. F , TPM expression of Serpina3n , Ctsd , Lgals3 , and Gpnmb in OLs, Astro, and MG from MOG/EAE and CFA mice. Serpina3n is specifically upregulated in OL, while Ctsd , Lgals3 , and Gpnmb are enriched and upregulated in MG.
    O4 Apc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rat  (Miltenyi Biotec)
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    A , experimental design. Inflammatory demyelination of experimental autoimmune encephalomyelitis (EAE) was induced in adult mice via MOG immunization (CFA as control) at day 0 (D0), with pertussis administered i.p. on D0 and D2. RNA-seq was performed on whole spinal cords ( B ) and MACS-purified glial populations ( C-F ) at D30-D35. OLs, oligodendrocytes; Astro, astrocytes; MG, microglia. B , volcano plot of differentially expressed genes (DEGs) in spinal cord of MOG/EAE (n=4) vs. CFA mice (n=3) at D35. Top10 DEGs are labeled with gene symbols and FPKM values. C , schematic of magnetic beads-assisted cell sorting (MACS) of spinal cord glia at D30. OL, Astro, and myeloid cells (including MG) were isolated using <t>anti-O4,</t> anti-ACSA2, and anti-CD11b magnetic beads, respectively. D , purity validation of sorted population using representative markers Plp1 (OL), Tmem119 (MG), and Aldh1l1 (Astro). Expression is shown as TPM (transcripts per million). E , volcano plot of DEGs in MOG_OL versus CFA_OL. Serpina3n is highlighted in red. F , TPM expression of Serpina3n , Ctsd , Lgals3 , and Gpnmb in OLs, Astro, and MG from MOG/EAE and CFA mice. Serpina3n is specifically upregulated in OL, while Ctsd , Lgals3 , and Gpnmb are enriched and upregulated in MG.
    Rat, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    A , experimental design. Inflammatory demyelination of experimental autoimmune encephalomyelitis (EAE) was induced in adult mice via MOG immunization (CFA as control) at day 0 (D0), with pertussis administered i.p. on D0 and D2. RNA-seq was performed on whole spinal cords ( B ) and MACS-purified glial populations ( C-F ) at D30-D35. OLs, oligodendrocytes; Astro, astrocytes; MG, microglia. B , volcano plot of differentially expressed genes (DEGs) in spinal cord of MOG/EAE (n=4) vs. CFA mice (n=3) at D35. Top10 DEGs are labeled with gene symbols and FPKM values. C , schematic of magnetic beads-assisted cell sorting (MACS) of spinal cord glia at D30. OL, Astro, and myeloid cells (including MG) were isolated using anti-O4, anti-ACSA2, and anti-CD11b magnetic beads, respectively. D , purity validation of sorted population using representative markers Plp1 (OL), Tmem119 (MG), and Aldh1l1 (Astro). Expression is shown as TPM (transcripts per million). E , volcano plot of DEGs in MOG_OL versus CFA_OL. Serpina3n is highlighted in red. F , TPM expression of Serpina3n , Ctsd , Lgals3 , and Gpnmb in OLs, Astro, and MG from MOG/EAE and CFA mice. Serpina3n is specifically upregulated in OL, while Ctsd , Lgals3 , and Gpnmb are enriched and upregulated in MG.

    Journal: bioRxiv

    Article Title: Injury-transduced SerpinOLs modulate neuroinflammation and glial activation in the diseased and non-diseased CNS

    doi: 10.64898/2026.01.29.702669

    Figure Lengend Snippet: A , experimental design. Inflammatory demyelination of experimental autoimmune encephalomyelitis (EAE) was induced in adult mice via MOG immunization (CFA as control) at day 0 (D0), with pertussis administered i.p. on D0 and D2. RNA-seq was performed on whole spinal cords ( B ) and MACS-purified glial populations ( C-F ) at D30-D35. OLs, oligodendrocytes; Astro, astrocytes; MG, microglia. B , volcano plot of differentially expressed genes (DEGs) in spinal cord of MOG/EAE (n=4) vs. CFA mice (n=3) at D35. Top10 DEGs are labeled with gene symbols and FPKM values. C , schematic of magnetic beads-assisted cell sorting (MACS) of spinal cord glia at D30. OL, Astro, and myeloid cells (including MG) were isolated using anti-O4, anti-ACSA2, and anti-CD11b magnetic beads, respectively. D , purity validation of sorted population using representative markers Plp1 (OL), Tmem119 (MG), and Aldh1l1 (Astro). Expression is shown as TPM (transcripts per million). E , volcano plot of DEGs in MOG_OL versus CFA_OL. Serpina3n is highlighted in red. F , TPM expression of Serpina3n , Ctsd , Lgals3 , and Gpnmb in OLs, Astro, and MG from MOG/EAE and CFA mice. Serpina3n is specifically upregulated in OL, while Ctsd , Lgals3 , and Gpnmb are enriched and upregulated in MG.

    Article Snippet: After centrifugation, the supernatant was gently removed, and the brain homogenate pellet was incubated separately with anti-CD11b MicroBeads (Cat# 130-093-634, Miltenyi Biotec, Germany) and anti-O4 MicroBeads (Cat# 130-094-543, Miltenyi Biotec, Germany).

    Techniques: Control, RNA Sequencing, Purification, Labeling, Magnetic Beads, FACS, Isolation, Biomarker Discovery, Expressing